human nasal epithelial cell line hnepc  (Procell Inc)

Journal: Scientific Reports

Article Title: Immuno-transcriptomic analysis based on machine learning identifies immunity signature genes of chronic rhinosinusitis with nasal polyps

doi: 10.1038/s41598-025-02508-8

Figure Lengend Snippet: Effects of CXCR1 and PPBP on IL-8 secretion. ( A ) HNEpC cells were transfected with siRNA (targeting CXCR1), and CXCR1 expression in transfected cells was detected by RT-qPCR. ( B ) Detection of CXCR1 protein expression by western blot. ( C ) Detection of IL-8 in the supernatant of CXCR1 knockdown cells using an ELISA kit. ( D ) HNEpC cells were transfected with PPBP overexpression plasmid and PPBP expression was detected by RT-qPCR in the transfected cells. ( E ) PPBP protein expression was detected by western blot. ( F ) The level of IL-8 in the supernatant of PPBP overexpressing cells was detected using ELISA kit.

Article Snippet: The human nasal epithelial cell line (HNEpC) was obtained from Procell (Catalogue No. CP-H252) and cultured in Human Nasal Mucosal Epithelial Cell Complete Medium (No. CM-H252), and the cells were incubated in an incubator with 5% CO2 at 37 °C.

Techniques: Transfection, Expressing, Quantitative RT-PCR, Western Blot, Knockdown, Enzyme-linked Immunosorbent Assay, Over Expression, Plasmid Preparation

Journal: International Journal of Molecular Sciences

Article Title: Triterpenoid Saponins and Flavonoid Glycosides from the Flower of Camellia flavida and Their Cytotoxic and α-Glycosidase Inhibitory Activities

doi: 10.3390/ijms252010977

Figure Lengend Snippet: Cytotoxicity (IC 50 , μM ± SD, n = 8) of compounds 1 – 10 against cell lines.

Article Snippet: The human cervical cancer cell line Hela, the human lung adenocarcinoma cell line A549, the human breast cancer cell lines MCF-7 and MDA-MB-231, the human hepatoma cell lines BEL-7402 and Hep G2, the human embryonic lung fibroblast cell line MRC-5, and the human nasal epithelial cell line HNEpC were all sourced from the China Center for Type Culture Collection (CCTCC, Wuhan University, Hubei, China).

Techniques:

Journal: Scientific Reports

Article Title: Insights from bioinformatics analysis reveal that lipopolysaccharide induces activation of chemokine-related signaling pathways in human nasal epithelial cells

doi: 10.1038/s41598-024-58317-y

Figure Lengend Snippet: ( A ) Cell viability of HNEpC treated with LPS at concentrations of 0.125, 0.25, 0.5, 1, 2, and 4 μg/mL for 24 h. ( B ) A PCA plot revealed a distinct separation between the LPS intervention group and control group. DEGs between HNEpC treated with LPS (1 μg/mL) or not ( C – E ). ( C ) Correlation scatter diagram of DEGs. ( D ) Volcano plot of DEGs. ( E ) Clustered heatmap of DEGs. ( F ) Heatmap displaying the top 50 significant DEGs, including 30 upregulated DEGs and 20 downregulated DEGs.

Article Snippet: First, to obtain a stable baseline of mRNA expression for RNA sequencing and avoid heterogeneity which may confuse the effects of LPS stimulation, we chose a commercial cell line HNEpC cell (PromoCell) with greater stability, less genetic variation and heterogeneity, and higher repeatability than primary epithelial cells directly isolated from human or animal tissues for cell culture and stimulation experiment.

Techniques: Control

Journal: Scientific Reports

Article Title: Insights from bioinformatics analysis reveal that lipopolysaccharide induces activation of chemokine-related signaling pathways in human nasal epithelial cells

doi: 10.1038/s41598-024-58317-y

Figure Lengend Snippet: GSEA and PPI network of HNEpC treated with LPS or not. ( A ) Barplot of NES of pathways significantly enriched in HNEpC treated with LPS or not. ( B ) GSEA enrichment plot of IL-17 signaling pathway, TNF signaling pathway, and chemokine signaling pathway in HNEpC treated with LPS or not. ( C ) PPI network of DEGs constructed by Cytoscape software. ( D ) The top 22 hub genes based on the closeness parameter calculated by Cytoscape software.

Article Snippet: First, to obtain a stable baseline of mRNA expression for RNA sequencing and avoid heterogeneity which may confuse the effects of LPS stimulation, we chose a commercial cell line HNEpC cell (PromoCell) with greater stability, less genetic variation and heterogeneity, and higher repeatability than primary epithelial cells directly isolated from human or animal tissues for cell culture and stimulation experiment.

Techniques: Construct, Software

Journal: Scientific Reports

Article Title: Insights from bioinformatics analysis reveal that lipopolysaccharide induces activation of chemokine-related signaling pathways in human nasal epithelial cells

doi: 10.1038/s41598-024-58317-y

Figure Lengend Snippet: IPA of the LPS treated and control HNEpC dataset. ( A ) Histogram of the most significantly enriched ingenuity canonical pathways (− log10 ( p -value) > 2.5). The number in the right side of the figure represents the number of all genes in this pathway, and the length of the red bar represents the percentage of upregulated (red) or downregulated (green) genes in this pathway identified through IPA analysis on the HNEpC dataset. ( B ) Top 10 ingenuity canonical pathways with − log10 ( p -value), Ratio, and Molecules. The ratio represents the ratio of the number of molecules to the total number of molecules in this pathway. ( C ) Bubble plot indicating that neurotransmitters and other nervous system signaling, pathogen-influenced signaling, cellular immune response, disease-specific pathways, and cytokine signaling pathways were pathways with the highest z -score and the largest number of genes.

Article Snippet: First, to obtain a stable baseline of mRNA expression for RNA sequencing and avoid heterogeneity which may confuse the effects of LPS stimulation, we chose a commercial cell line HNEpC cell (PromoCell) with greater stability, less genetic variation and heterogeneity, and higher repeatability than primary epithelial cells directly isolated from human or animal tissues for cell culture and stimulation experiment.

Techniques: Control

Journal: Scientific Reports

Article Title: Insights from bioinformatics analysis reveal that lipopolysaccharide induces activation of chemokine-related signaling pathways in human nasal epithelial cells

doi: 10.1038/s41598-024-58317-y

Figure Lengend Snippet: ( A ) Key upstream regulators (CG, IL17A, IL1A, IL1B, IL1RAP, IL1RL2, IL36A, IL36G, JUN, NFkB (complex), NPM1, STAT3, TNF) and target molecules (BIRC3, CCL20, CSF1, CSF3, CXCL1, CXCL10, CXCL8, NFKBIZ, SAA1) in the LPS treated and control HNEpC dataset. ( B ) Detailed information on upstream regulators in the LPS treated and control HNEpC dataset. ( C ) Graphical summary of the LPS treated and control HNEpC dataset.

Article Snippet: First, to obtain a stable baseline of mRNA expression for RNA sequencing and avoid heterogeneity which may confuse the effects of LPS stimulation, we chose a commercial cell line HNEpC cell (PromoCell) with greater stability, less genetic variation and heterogeneity, and higher repeatability than primary epithelial cells directly isolated from human or animal tissues for cell culture and stimulation experiment.

Techniques: Control

Journal: Scientific Reports

Article Title: Insights from bioinformatics analysis reveal that lipopolysaccharide induces activation of chemokine-related signaling pathways in human nasal epithelial cells

doi: 10.1038/s41598-024-58317-y

Figure Lengend Snippet: Validation of genes in chemokines related signaling pathways by qRT-PCR. The relative expression levels of CXCL1, CXCL8, CXCL10, CCL20, RELB, LCN2, BIRC3, NFKBIZ, IRAK2, PI3, SAA1, CSF3, and CSF1 in HNEpC ( A ) or pHNEpC ( B ) treated with LPS or not. GAPDH was used as a reference. Each group contained three biological sample repeats, and each sample contained three technical repeats of qRT-PCR.

Article Snippet: First, to obtain a stable baseline of mRNA expression for RNA sequencing and avoid heterogeneity which may confuse the effects of LPS stimulation, we chose a commercial cell line HNEpC cell (PromoCell) with greater stability, less genetic variation and heterogeneity, and higher repeatability than primary epithelial cells directly isolated from human or animal tissues for cell culture and stimulation experiment.

Techniques: Quantitative RT-PCR, Expressing

Journal: Scientific Reports

Article Title: Insights from bioinformatics analysis reveal that lipopolysaccharide induces activation of chemokine-related signaling pathways in human nasal epithelial cells

doi: 10.1038/s41598-024-58317-y

Figure Lengend Snippet: Schematic diagram displaying a hypothetical chain of events in which LPS intervention could markedly change the transcriptome of HNEpC, and then LPS could trigger inflammatory responses of HNEpC by regulating the expression of key genes in chemokines related signaling pathways.

Article Snippet: First, to obtain a stable baseline of mRNA expression for RNA sequencing and avoid heterogeneity which may confuse the effects of LPS stimulation, we chose a commercial cell line HNEpC cell (PromoCell) with greater stability, less genetic variation and heterogeneity, and higher repeatability than primary epithelial cells directly isolated from human or animal tissues for cell culture and stimulation experiment.

Techniques: Expressing

Journal: Balkan Medical Journal

Article Title: Silencing SOX11 Alleviates Allergic Rhinitis by Inhibiting Epithelial-Derived Cytokines

doi: 10.4274/balkanmedj.galenos.2022.2022-9-31

Figure Lengend Snippet: STAT6 enhanced the transcription of SOX11 gene. (a) Schematic model of predicted binding sites of STAT6 on SOX11 promoter. (b) Effects of STAT6 on the transcriptional activity of truncated SOX11 promoter fragments. (c) SOX11 expression in AS1517499-treated HNEpCs measured by qRT-PCR. (d) SOX11 protein levels in AS1517499-treated HNEpCs obtained by Western blotting analysis. ** P < 0.01 vs. SOX11 promoter (−1757 to +20) + Vector-OE group; ## P < 0.01 vs. SOX11 promoter (−1757 to +20) + STAT6-OE group; §§ P < 0.01 vs. SOX11 promoter (−1334 to +20) + STAT6-OE group.

Article Snippet: The human nasal mucosa epithelial cell line HNEpC was procured from iCell Bioscience (Shanghai, China).

Techniques: Binding Assay, Activity Assay, Expressing, Quantitative RT-PCR, Western Blot, Plasmid Preparation

Journal: Balkan Medical Journal

Article Title: Silencing SOX11 Alleviates Allergic Rhinitis by Inhibiting Epithelial-Derived Cytokines

doi: 10.4274/balkanmedj.galenos.2022.2022-9-31

Figure Lengend Snippet: SOX11 and STAT6 expression were upregulated in IL-13-treated HNEpCs. (a) Schematic diagram of the culture and treatment of HNEpCs. (b) SOX11 expression measured by qRT-PCR. (c) SOX11 protein expression determined by Western blotting. (d) Immunofluorescence staining for SOX11 expression. (e) Representative images and relative protein levels of p-STAT6 and STAT6 obtained by Western blotting analysis.

Article Snippet: The human nasal mucosa epithelial cell line HNEpC was procured from iCell Bioscience (Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Immunofluorescence, Staining

Journal: Balkan Medical Journal

Article Title: Silencing SOX11 Alleviates Allergic Rhinitis by Inhibiting Epithelial-Derived Cytokines

doi: 10.4274/balkanmedj.galenos.2022.2022-9-31

Figure Lengend Snippet: Silencing SOX11 reduced the epithelial-derived cytokine expression and mucin level in IL-13-treated HNEpCs. (a) SOX11 expression in IL-13-induced HNEpCs measured by qRT-PCR. (b) SOX11 protein levels obtained by Western blotting analysis. (c-f) Expression of epithelial-derived cytokines measured by qRT-PCR. (g) MUC5AC levels in cell supernatants detected by ELISA.

Article Snippet: The human nasal mucosa epithelial cell line HNEpC was procured from iCell Bioscience (Shanghai, China).

Techniques: Derivative Assay, Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Downregulation of lncRNA X Inactive Specific Transcript (XIST) Suppresses Cell Proliferation and Enhances Radiosensitivity by Upregulating mir-29c in Nasopharyngeal Carcinoma Cells

doi: 10.12659/MSM.905370

Figure Lengend Snippet: Expression alteration of XIST and miR-29c in NPC cells in response to irradiation. ( A ) qRT-PCR was performed to examine the expressions of XIST and miR-29c in NPC cell lines (CNE1 and CNE2) and primary normal human nasal epithelial line HNEpC. qRT-PCR was carried out to analyze the expressions of XIST ( B ) and miR-29c (C) in CNE1 and CNE2 cells at indicated time points after 4-Gy irradiation treatment. * P <0.05.

Article Snippet: Primary normal human nasal epithelial line HNEpC was obtained from PromoCell (Heidelberg, Germany) and cultured with commercially available Airway Epithelial Cell Growth Medium (PromoCell) at 37°C under 5% CO 2 .

Techniques: Expressing, Irradiation, Quantitative RT-PCR